Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value.
Identifieur interne : 000E30 ( Main/Exploration ); précédent : 000E29; suivant : 000E31Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value.
Auteurs : David Veyer [France] ; Solen Kernéis [France] ; Geoffroy Poulet [France] ; Maxime Wack [France] ; Nicolas Robillard [France] ; Valérie Taly [France] ; Anne-Sophie L'Honneur [France] ; Flore Rozenberg [France] ; Pierre Laurent-Puig [France] ; Laurent Bélec [France] ; Jérôme Hadjadj [France] ; Benjamin Terrier [France] ; Hélène Péré [France]Source :
- Clinical infectious diseases : an official publication of the Infectious Diseases Society of America [ 1537-6591 ] ; 2020.
Abstract
BACKGROUND
Coronavirus disease 2019 (COVID-19) is a global public health problem that has already caused more than 662,000 deaths worldwide. Although the clinical manifestations of COVID-19 are dominated by respiratory symptoms, some patients present other severe damage such as cardiovascular, renal and liver injury or/and multiple organ failure, suggesting a spread of the SARS-CoV-2 in blood. Recent ultrasensitive polymerase chain reaction (PCR) technology now allows absolute quantification of nucleic acids in plasma. We herein intended to use the droplet-based digital PCR technology to obtain sensitive detection and precise quantification of plasma SARS-CoV-2 viral load (SARS-CoV-2 RNAaemia) in hospitalized COVID-19 patients.
METHODS
Fifty-eight consecutive COVID-19 patients with pneumonia 8 to 12 days after onset of symptoms and 12 healthy controls were analyzed. Disease severity was categorized as mild-to-moderate in 17 patients, severe in 16 patients and critical in 26 patients. Plasma SARS-CoV-2 RNAaemia was quantified by droplet digital Crystal Digital PCR™ next-generation technology (Stilla Technologies, Villejuif, France).
RESULTS
Overall, SARS-CoV-2 RNAaemia was detected in 43 (74.1%) patients. Prevalence of positive SARS-CoV-2 RNAaemia correlated with disease severity, ranging from 53% in mild-to-moderate patients to 88% in critically ill patients (p=0.036). Levels of SARS-CoV-2 RNAaemia were associated with severity (p=0.035). Among nine patients who experienced clinical deterioration during follow-up, eight had positive SARS-CoV-2 RNAaemia at baseline while only one critical patient with undetectable SARS-CoV-2 RNAaemia at the time of analysis died at day 27.
CONCLUSION
SARS-CoV-2 RNAaemia measured by droplet-based digital PCR constitutes a promising prognosis biomarker in COVID-19 patients.
DOI: 10.1093/cid/ciaa1196
PubMed: 32803231
PubMed Central: PMC7454373
Affiliations:
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wicri:level="3"><nlm:affiliation>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Hadjadj, Jerome" sort="Hadjadj, Jerome" uniqKey="Hadjadj J" first="Jérôme" last="Hadjadj">Jérôme Hadjadj</name><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Biochimie, Hôpital Européen Georges Pompidou, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de 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Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Pere, Helene" sort="Pere, Helene" uniqKey="Pere H" first="Hélène" last="Péré">Hélène Péré</name><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>PARCC, Université de Paris, INSERM U970, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>PARCC, Université de Paris, INSERM U970, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32803231</idno><idno type="pmid">32803231</idno><idno type="doi">10.1093/cid/ciaa1196</idno><idno type="pmc">PMC7454373</idno><idno type="wicri:Area/Main/Corpus">000582</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000582</idno><idno type="wicri:Area/Main/Curation">000582</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000582</idno><idno type="wicri:Area/Main/Exploration">000582</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value.</title><author><name sortKey="Veyer, David" sort="Veyer, David" uniqKey="Veyer D" first="David" last="Veyer">David Veyer</name><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Functional Genomics of Solid Tumors laboratory, équipe labellisée Ligue Nationale contre le Cancer, Labex OncoImmunology, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Functional Genomics of Solid Tumors laboratory, équipe labellisée Ligue Nationale contre le Cancer, Labex OncoImmunology, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Kerneis, Solen" sort="Kerneis, Solen" uniqKey="Kerneis S" first="Solen" last="Kernéis">Solen Kernéis</name><affiliation wicri:level="3"><nlm:affiliation>Equipe Mobile d'Infectiologie, AP-HP, APHP.CUP, Hôpital Cochin, F-75014 Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Equipe Mobile d'Infectiologie, AP-HP, APHP.CUP, Hôpital Cochin, F-75014 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Université de Paris, INSERM, IAME, F-75006 Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Université de Paris, INSERM, IAME, F-75006 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Poulet, Geoffroy" sort="Poulet, Geoffroy" uniqKey="Poulet G" first="Geoffroy" last="Poulet">Geoffroy Poulet</name><affiliation wicri:level="3"><nlm:affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Personalized Medicine Pharmacogenomics, therapeutic optimization, eDIAG plateform, laboratory, équipe labellisée Ligue Nationale contre le Cancer, Labex OncoImmunology, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Personalized Medicine Pharmacogenomics, therapeutic optimization, eDIAG plateform, laboratory, équipe labellisée Ligue Nationale contre le Cancer, Labex OncoImmunology, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Wack, Maxime" sort="Wack, Maxime" uniqKey="Wack M" first="Maxime" last="Wack">Maxime Wack</name><affiliation wicri:level="3"><nlm:affiliation>Eurofins-Biomnis, Lyon.</nlm:affiliation><country>France</country><placeName><settlement type="city">Lyon</settlement><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Rhône-Alpes</region></placeName><wicri:orgArea>Eurofins-Biomnis</wicri:orgArea></affiliation><affiliation wicri:level="3"><nlm:affiliation>Département d'Informatique Médicale, Biostatistiques et Santé Publique, Hôpital Européen Georges Pompidou, AP-HP CUP, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Département d'Informatique Médicale, Biostatistiques et Santé Publique, Hôpital Européen Georges Pompidou, AP-HP CUP, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Robillard, Nicolas" sort="Robillard, Nicolas" uniqKey="Robillard N" first="Nicolas" last="Robillard">Nicolas Robillard</name><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Taly, Valerie" sort="Taly, Valerie" uniqKey="Taly V" first="Valérie" last="Taly">Valérie Taly</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="L Honneur, Anne Sophie" sort="L Honneur, Anne Sophie" uniqKey="L Honneur A" first="Anne-Sophie" last="L'Honneur">Anne-Sophie L'Honneur</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Information sciences to support medicine Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Information sciences to support medicine Paris</wicri:regionArea><wicri:noRegion>Information sciences to support medicine Paris</wicri:noRegion><wicri:noRegion>Information sciences to support medicine Paris</wicri:noRegion></affiliation></author><author><name sortKey="Rozenberg, Flore" sort="Rozenberg, Flore" uniqKey="Rozenberg F" first="Flore" last="Rozenberg">Flore Rozenberg</name><affiliation wicri:level="1"><nlm:affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Information sciences to support medicine Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Information sciences to support medicine Paris</wicri:regionArea><wicri:noRegion>Information sciences to support medicine Paris</wicri:noRegion><wicri:noRegion>Information sciences to support medicine Paris</wicri:noRegion></affiliation></author><author><name sortKey="Laurent Puig, Pierre" sort="Laurent Puig, Pierre" uniqKey="Laurent Puig P" first="Pierre" last="Laurent-Puig">Pierre Laurent-Puig</name><affiliation wicri:level="3"><nlm:affiliation>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris</wicri:regionArea><placeName><region type="region" nuts="2">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Virologie, Hôpital Cochin, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Virologie, Hôpital Cochin, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Belec, Laurent" sort="Belec, Laurent" uniqKey="Belec L" first="Laurent" last="Bélec">Laurent Bélec</name><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Hadjadj, Jerome" sort="Hadjadj, Jerome" uniqKey="Hadjadj J" first="Jérôme" last="Hadjadj">Jérôme Hadjadj</name><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Biochimie, Hôpital Européen Georges Pompidou, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Biochimie, Hôpital Européen Georges Pompidou, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation><nlm:affiliation>Imagine Institute, laboratory of Immunogenetics of Pediatric Autoimmune Diseases, INSERM UMR 1163.</nlm:affiliation><wicri:noCountry code="subField">INSERM UMR 1163</wicri:noCountry></affiliation></author><author><name sortKey="Terrier, Benjamin" sort="Terrier, Benjamin" uniqKey="Terrier B" first="Benjamin" last="Terrier">Benjamin Terrier</name><affiliation><nlm:affiliation>Imagine Institute, laboratory of Immunogenetics of Pediatric Autoimmune Diseases, INSERM UMR 1163.</nlm:affiliation><wicri:noCountry code="subField">INSERM UMR 1163</wicri:noCountry></affiliation><affiliation wicri:level="3"><nlm:affiliation>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author><author><name sortKey="Pere, Helene" sort="Pere, Helene" uniqKey="Pere H" first="Hélène" last="Péré">Hélène Péré</name><affiliation wicri:level="3"><nlm:affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation><affiliation wicri:level="3"><nlm:affiliation>PARCC, Université de Paris, INSERM U970, Paris, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>PARCC, Université de Paris, INSERM U970, Paris</wicri:regionArea><placeName><region type="region">Île-de-France</region><region type="old region">Île-de-France</region><settlement type="city">Paris</settlement></placeName></affiliation></author></analytic><series><title level="j">Clinical infectious diseases : an official publication of the Infectious Diseases Society of America</title><idno type="eISSN">1537-6591</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Coronavirus disease 2019 (COVID-19) is a global public health problem that has already caused more than 662,000 deaths worldwide. Although the clinical manifestations of COVID-19 are dominated by respiratory symptoms, some patients present other severe damage such as cardiovascular, renal and liver injury or/and multiple organ failure, suggesting a spread of the SARS-CoV-2 in blood. Recent ultrasensitive polymerase chain reaction (PCR) technology now allows absolute quantification of nucleic acids in plasma. We herein intended to use the droplet-based digital PCR technology to obtain sensitive detection and precise quantification of plasma SARS-CoV-2 viral load (SARS-CoV-2 RNAaemia) in hospitalized COVID-19 patients.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Fifty-eight consecutive COVID-19 patients with pneumonia 8 to 12 days after onset of symptoms and 12 healthy controls were analyzed. Disease severity was categorized as mild-to-moderate in 17 patients, severe in 16 patients and critical in 26 patients. Plasma SARS-CoV-2 RNAaemia was quantified by droplet digital Crystal Digital PCR™ next-generation technology (Stilla Technologies, Villejuif, France).</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Overall, SARS-CoV-2 RNAaemia was detected in 43 (74.1%) patients. Prevalence of positive SARS-CoV-2 RNAaemia correlated with disease severity, ranging from 53% in mild-to-moderate patients to 88% in critically ill patients (p=0.036). Levels of SARS-CoV-2 RNAaemia were associated with severity (p=0.035). Among nine patients who experienced clinical deterioration during follow-up, eight had positive SARS-CoV-2 RNAaemia at baseline while only one critical patient with undetectable SARS-CoV-2 RNAaemia at the time of analysis died at day 27.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>SARS-CoV-2 RNAaemia measured by droplet-based digital PCR constitutes a promising prognosis biomarker in COVID-19 patients.</p></div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32803231</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>01</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1537-6591</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Aug</Month><Day>17</Day></PubDate></JournalIssue><Title>Clinical infectious diseases : an official publication of the Infectious Diseases Society of America</Title><ISOAbbreviation>Clin. Infect. Dis.</ISOAbbreviation></Journal><ArticleTitle>Highly sensitive quantification of plasma SARS-CoV-2 RNA shelds light on its potential clinical value.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">ciaa1196</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/cid/ciaa1196</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Coronavirus disease 2019 (COVID-19) is a global public health problem that has already caused more than 662,000 deaths worldwide. Although the clinical manifestations of COVID-19 are dominated by respiratory symptoms, some patients present other severe damage such as cardiovascular, renal and liver injury or/and multiple organ failure, suggesting a spread of the SARS-CoV-2 in blood. Recent ultrasensitive polymerase chain reaction (PCR) technology now allows absolute quantification of nucleic acids in plasma. We herein intended to use the droplet-based digital PCR technology to obtain sensitive detection and precise quantification of plasma SARS-CoV-2 viral load (SARS-CoV-2 RNAaemia) in hospitalized COVID-19 patients.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Fifty-eight consecutive COVID-19 patients with pneumonia 8 to 12 days after onset of symptoms and 12 healthy controls were analyzed. Disease severity was categorized as mild-to-moderate in 17 patients, severe in 16 patients and critical in 26 patients. Plasma SARS-CoV-2 RNAaemia was quantified by droplet digital Crystal Digital PCR™ next-generation technology (Stilla Technologies, Villejuif, France).</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Overall, SARS-CoV-2 RNAaemia was detected in 43 (74.1%) patients. Prevalence of positive SARS-CoV-2 RNAaemia correlated with disease severity, ranging from 53% in mild-to-moderate patients to 88% in critically ill patients (p=0.036). Levels of SARS-CoV-2 RNAaemia were associated with severity (p=0.035). Among nine patients who experienced clinical deterioration during follow-up, eight had positive SARS-CoV-2 RNAaemia at baseline while only one critical patient with undetectable SARS-CoV-2 RNAaemia at the time of analysis died at day 27.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">SARS-CoV-2 RNAaemia measured by droplet-based digital PCR constitutes a promising prognosis biomarker in COVID-19 patients.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press for the Infectious Diseases Society of America. All rights reserved. For permissions, e-mail: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Veyer</LastName><ForeName>David</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Functional Genomics of Solid Tumors laboratory, équipe labellisée Ligue Nationale contre le Cancer, Labex OncoImmunology, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kernéis</LastName><ForeName>Solen</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Equipe Mobile d'Infectiologie, AP-HP, APHP.CUP, Hôpital Cochin, F-75014 Paris, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Université de Paris, INSERM, IAME, F-75006 Paris, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Poulet</LastName><ForeName>Geoffroy</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Personalized Medicine Pharmacogenomics, therapeutic optimization, eDIAG plateform, laboratory, équipe labellisée Ligue Nationale contre le Cancer, Labex OncoImmunology, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wack</LastName><ForeName>Maxime</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Eurofins-Biomnis, Lyon.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Département d'Informatique Médicale, Biostatistiques et Santé Publique, Hôpital Européen Georges Pompidou, AP-HP CUP, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Robillard</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Taly</LastName><ForeName>Valérie</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>L'Honneur</LastName><ForeName>Anne-Sophie</ForeName><Initials>AS</Initials><AffiliationInfo><Affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Information sciences to support medicine Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Rozenberg</LastName><ForeName>Flore</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Centre de Recherche des Cordeliers, Sorbonne Université, Inserm, Université de Paris, Information sciences to support medicine Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laurent-Puig</LastName><ForeName>Pierre</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Institut Pasteur, Epidemiology and Modelling of Antibiotic Evasion (EMAE), F-75015 Paris, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Virologie, Hôpital Cochin, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bélec</LastName><ForeName>Laurent</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hadjadj</LastName><ForeName>Jérôme</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Biochimie, Hôpital Européen Georges Pompidou, Paris, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Imagine Institute, laboratory of Immunogenetics of Pediatric Autoimmune Diseases, INSERM UMR 1163.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Terrier</LastName><ForeName>Benjamin</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Imagine Institute, laboratory of Immunogenetics of Pediatric Autoimmune Diseases, INSERM UMR 1163.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Internal Medicine, National Referral Center for Rare Systemic Autoimmune Diseases, AP-HP CUP, Paris, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Péré</LastName><ForeName>Hélène</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Assistance Publique Hôpitaux de Paris-Centre (AP-HP.Centre), Service de Microbiologie (Unité de virologie), Hôpital Européen Georges Pompidou, Paris, France.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>PARCC, Université de Paris, INSERM U970, Paris, France.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>08</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Clin Infect Dis</MedlineTA><NlmUniqueID>9203213</NlmUniqueID><ISSNLinking>1058-4838</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>05</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>8</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>8</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>8</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32803231</ArticleId><ArticleId IdType="pii">5893132</ArticleId><ArticleId IdType="doi">10.1093/cid/ciaa1196</ArticleId><ArticleId IdType="pmc">PMC7454373</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>France</li></country><region><li>Auvergne-Rhône-Alpes</li><li>Rhône-Alpes</li><li>Île-de-France</li></region><settlement><li>Lyon</li><li>Paris</li></settlement></list><tree><country name="France"><region name="Île-de-France"><name sortKey="Veyer, David" sort="Veyer, David" uniqKey="Veyer D" first="David" last="Veyer">David Veyer</name></region><name sortKey="Belec, Laurent" sort="Belec, Laurent" uniqKey="Belec L" first="Laurent" last="Bélec">Laurent Bélec</name><name sortKey="Belec, Laurent" sort="Belec, Laurent" uniqKey="Belec L" first="Laurent" last="Bélec">Laurent Bélec</name><name sortKey="Hadjadj, Jerome" sort="Hadjadj, Jerome" uniqKey="Hadjadj J" first="Jérôme" last="Hadjadj">Jérôme Hadjadj</name><name sortKey="Kerneis, Solen" sort="Kerneis, Solen" uniqKey="Kerneis S" first="Solen" last="Kernéis">Solen Kernéis</name><name sortKey="Kerneis, Solen" sort="Kerneis, Solen" uniqKey="Kerneis S" first="Solen" last="Kernéis">Solen Kernéis</name><name sortKey="Kerneis, Solen" sort="Kerneis, Solen" uniqKey="Kerneis S" first="Solen" last="Kernéis">Solen Kernéis</name><name sortKey="L Honneur, Anne Sophie" sort="L Honneur, Anne Sophie" uniqKey="L Honneur A" first="Anne-Sophie" last="L'Honneur">Anne-Sophie L'Honneur</name><name sortKey="Laurent Puig, Pierre" sort="Laurent Puig, Pierre" uniqKey="Laurent Puig P" first="Pierre" last="Laurent-Puig">Pierre Laurent-Puig</name><name sortKey="Laurent Puig, Pierre" sort="Laurent Puig, Pierre" uniqKey="Laurent Puig P" first="Pierre" last="Laurent-Puig">Pierre Laurent-Puig</name><name sortKey="Pere, Helene" sort="Pere, Helene" uniqKey="Pere H" first="Hélène" last="Péré">Hélène Péré</name><name sortKey="Pere, Helene" sort="Pere, Helene" uniqKey="Pere H" first="Hélène" last="Péré">Hélène Péré</name><name sortKey="Poulet, Geoffroy" sort="Poulet, Geoffroy" uniqKey="Poulet G" first="Geoffroy" last="Poulet">Geoffroy Poulet</name><name sortKey="Robillard, Nicolas" sort="Robillard, Nicolas" uniqKey="Robillard N" first="Nicolas" last="Robillard">Nicolas Robillard</name><name sortKey="Rozenberg, Flore" sort="Rozenberg, Flore" uniqKey="Rozenberg F" first="Flore" last="Rozenberg">Flore Rozenberg</name><name sortKey="Taly, Valerie" sort="Taly, Valerie" uniqKey="Taly V" first="Valérie" last="Taly">Valérie Taly</name><name sortKey="Terrier, Benjamin" sort="Terrier, Benjamin" uniqKey="Terrier B" first="Benjamin" last="Terrier">Benjamin Terrier</name><name sortKey="Veyer, David" sort="Veyer, David" uniqKey="Veyer D" first="David" last="Veyer">David Veyer</name><name sortKey="Wack, Maxime" sort="Wack, Maxime" uniqKey="Wack M" first="Maxime" last="Wack">Maxime Wack</name><name sortKey="Wack, Maxime" sort="Wack, Maxime" uniqKey="Wack M" first="Maxime" last="Wack">Maxime Wack</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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